›› 2009, Vol. 40 ›› Issue (4): 585-589.doi: 10.3969/j.issn.0529-1356.2009.04.013

• 论著 • 上一篇    下一篇

雌激素具有抗血管平滑肌细胞应激性衰老效应

朱从丽;郑勇;徐菁; 宋健*   

  1. 武汉大学医学院人体解剖学与组织学胚胎学系,武汉 430071
  • 收稿日期:2008-06-30 修回日期:2008-08-20 出版日期:2009-08-06
  • 通讯作者: 宋健

Estrogen exerts the effect on stressinduced senescence of vascular smooth muscl cells EM>in vitro/EM>

  1. Department of Anatomy, Histology and Embryology, Wuhan University School of Medicine, Wuhan 430071, China
  • Received:2008-06-30 Revised:2008-08-20 Online:2009-08-06
  • Contact: SONG Jian

关键词: 雌激素, 血管平滑肌细胞, 应激性衰老, Ras, p21SUP>WAF1, /SUP>, 细胞化学, Pull down assay, 大鼠

Abstract: Objective To explore the effects of estrogen on stressinduced senescence of vascular smooth muscle cells (VSMCs) and the underlying mechanisms. Methods The VSMCs of passage 23 cultured from female SD rats were induced into senescence by exposing to 150μmol/L HSUB>2/SUB>OSUB>2/SUB> in the presence or absence of different concentrations(10SUP>-10/SUP>mol/L10SUP>-8/SUP>mol/L) of 17β-estradiol (ESUB>2/SUB>). The expressions or activities of senescence associated marker DcR2, senescence-ssociated beta-galactosidase (SA-β-Gal), oncogene Ras and p21SUP>WAF1/SUP> were detected by flow cytometry, cytochemical staining, pulldown assay or Western blotting analysis. Results Flow cytometry analysis showed that in the physiological concentrations, ESUB>2/SUB> significantly inhibited the HSUB>2/SUB> OSUB>2/SUB>promoted highlevel expression of DcR2 of VSMCs in a dosedependent manner, with a highest inhibitive rate at 14.48%±0.6%(ESUB>2/SUB>=10SUP>8/SUP>mol/L;EM>P/EM><0.05, EM>n/EM>=3); this inhibitive effect could be blocked by a ESUB>2/SUB> receptors inhibitor ICI 182,780. Cytochemistry staining showed that the rate of SA-β-Gal positive VSMCs induced by HSUB>2/SUB>OSUB>2/SUB> decreased in presence of 10SUP>-8/SUP>mol/L ESUB>2/SUB> (20.5%±1.4% vs 9.6%±0.9%;EM>P/EM><0.05, EM>n/EM>=9). Pulldown assay and Western blotting analysis revealed that administration of 10SUP>-8/SUP>mol/L ESUB>2/SUB> obviously reduced the HSUB>2/SUB>OSUB>2/SUB>induced

Key words: Estrogen, Vascular smooth muscle cells, Stress inducedsenescence, Ras, p21SUP>WAF1/SUP>, Cytochemistry, Pulldown assay, Rat

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